LOCUS       40924_23704       12187 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB711 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12187)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12187)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12187)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12187)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1340..1370
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1424..2080
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2425..2727
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2771..2895)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2906..2929
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     terminator      2951..3203
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3237..3253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3261..3277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3285..3315)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3330..3351)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3387..3639)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3677..4972)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61190.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(3677..4972)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5024..5203)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5709..5733)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6254..7042
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7291..7879
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8065..8205)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8549..8743)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8812..9882)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10314..10940)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcgacta caaggatgac gatgacaagg cttagagctc
     2941 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     3001 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     3061 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     3121 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     3181 ggtgtcatct atgttactag atcgggaatt ggttccggaa ccaattcgta atcatggtca
     3241 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3301 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aggctgaatt
     3361 aggcgcgcct atttctatta attcccgatc tagtaacata gatgacaccg cgcgcgataa
     3421 tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg
     3481 gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac
     3541 atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt
     3601 caatcttaag aaactttatt gccaaatgtt tgaacgatcg gggaaattcg cctcgagtga
     3661 cttctctgat tgcagactat ttataccaac cagcaagaaa gtgtcgaagt atgaagcaga
     3721 aggcgcaggc tagagcctgg aaaacgagaa gatggatgca aagagacttt tctgatttag
     3781 gaccaaacaa cctcaagtaa acattgacga gtgtcccatc ttttgtgccg gtcagtaatc
     3841 cggtagcagg atcatacctt gggagacgat ggcgcggaca tttcacaatc ccagcaagct
     3901 gaggaagcga taatagaagg ttcagaactt gaggaagaaa gaagatgagg agggtttcac
     3961 tgaagtgacc cagaatacca acaactgcca tagtcattcc ggcgaagact gtgtaagtgt
     4021 ctccaacaaa aactgaagaa ggataccaat tgtaagcaag cattgccaag gatgtggcca
     4081 tcagcggttg agtaagaaat atcgagaagg catgagcttg gtgatactcg ggatcaacag
     4141 atgctccgat ttgcataacg ttgtgtatca ggatagcagc ggcaataaca actgtttgac
     4201 cgatttcaag gccattcaga ccagcgtgga tgttaataga gtttgtacaa aagacagcaa
     4261 gtagccccat atataactta tatatccttc ctagattaag cacttccaag ccaatataag
     4321 caacaagagg ttttggtata acaatagttg tatgaccagc ataagccatc aaaagtggaa
     4381 gggtagcaaa agatggcagg acaagtttta ctctccaagg cacatcaagg acatcatcta
     4441 caaatccaag caaaatcatg aagcaaatag atgctagtgc tgcattatac tccacaagcc
     4501 aattagaatc ttcagtgaag ttaaaatact gaaatattat cgccacgatc aaaaagacaa
     4561 taccaacaac aatacccaat gactctggca ctttaatatc tccttgaggc gtgcctcttt
     4621 tgttgatatc aaaaccaaac atgttacgtc tcagaacgta tctggcagta acaggaatca
     4681 ttttgagagt gacgaaaaac ccaaccaagc taagccccgc gttaattaga atcgatcgct
     4741 tgagttcatt atcaaccttg tagtgaaaac agagaaggta tagatagagc gaacagagaa
     4801 gagtagagat cacgaagatc actcctaatt tcggcggcgc gagacgaaat tcctcaccgg
     4861 aagcagaaac cgtctttcgg gtcatcttct gctccgatgg cgctgagttg ggttccgacg
     4921 ggtccggttt gttgggaatt gagatcgatg aagctctctt acgagctgcc atatcttatt
     4981 gccccccggg atcagatctg gattgagagt gaatatgaga ctctaattgg ataccgaggg
     5041 gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac
     5101 cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac
     5161 tccagaaacc cgcggctgag tggctccttc aacgttgcgg ttctgtcagt tccaaacgta
     5221 aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa ttctccgctc
     5281 atgatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     5341 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg
     5401 tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc
     5461 tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc
     5521 agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag cccttgcgcc
     5581 ctgagtgctt gcggcagcgt gaagctgatc cgtcgagatt aatagaaata aattcagcct
     5641 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
     5701 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
     5761 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
     5821 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
     5881 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     5941 aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc
     6001 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     6061 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     6121 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     6181 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     6241 aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     6301 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     6361 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     6421 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     6481 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     6541 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     6601 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     6661 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     6721 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     6781 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     6841 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     6901 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     6961 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     7021 gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct
     7081 tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa
     7141 ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt
     7201 gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7261 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7321 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7381 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     7441 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7501 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7561 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7621 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7681 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7741 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7801 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7861 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7921 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7981 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     8041 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     8101 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     8161 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     8221 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     8281 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     8341 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
     8401 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
     8461 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
     8521 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
     8581 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
     8641 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
     8701 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg
     8761 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
     8821 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
     8881 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
     8941 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
     9001 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
     9061 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
     9121 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
     9181 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
     9241 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
     9301 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
     9361 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
     9421 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
     9481 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
     9541 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
     9601 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
     9661 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
     9721 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
     9781 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
     9841 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
     9901 ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg
     9961 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
    10021 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
    10081 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
    10141 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
    10201 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
    10261 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
    10321 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
    10381 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
    10441 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
    10501 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
    10561 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
    10621 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
    10681 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
    10741 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
    10801 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
    10861 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
    10921 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
    10981 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
    11041 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
    11101 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
    11161 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
    11221 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
    11281 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
    11341 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
    11401 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
    11461 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
    11521 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
    11581 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
    11641 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
    11701 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
    11761 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
    11821 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
    11881 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
    11941 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    12001 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    12061 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    12121 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
    12181 taaacct
//
